Correlation between Warm Reactive Autoantibodies and the Monocyte Monolayer Assay
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چکیده
BACKGROUND: The monocyte monolayer assay (MMA) measures the adherence and phagocytosis of sensitized red cells with peripheral monocytes. The result of MMA may reflect the in vivo destruction of sensitized red blood cells (RBCs). STUDY DESIGN AND METHODS: Blood samples from 20 patients with warm reactive autoantibodies were investigated using the MMA. The Monocyte Index (MI) values > 5% were considered positive while MI values ≤ 5% were considered negative. RESULTS: Of the 20 patients, only one patient had a positive MMA result with an MI of 30%. All other 19 patients demonstrated a negative MI value (<5%). There was no correlation between serologically detectable warm reactive autoantibodies and the MMA or DAT. CONCLUSION: The final conclusion demands more comparative studies between the clinical and serologic features of patients with warm autoantibodies and the MMA tests. There are many variables that exist within the MMA which may have an effect on the outcome.
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